FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63359189
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12606550.079.461951
GTGTTGG12896250.075.9071962
TGTTGGG13179550.072.861363
GTTGGGT13900750.068.841864
TGGGTGT15148750.063.360886
TTGGGTG15377350.062.8271875
GGGTGTG15912250.060.2934387
GTTGCGT161500.059.5608864
TGTTGCG165150.057.7351153
GGTGTGT17039050.055.8853658
GTGTGTT17978600.053.1298079
TTGCGTG191500.050.9488645
TGCGTGT248250.040.6388176
GCGTGTG255900.040.382257
CGTGTGT270200.037.219058
TGTGTTT18016700.026.24989510-11
GTGTTTG20142100.025.28352410-11
GTGTCGG60300.024.6677952
TGTCGGG63200.023.2791293
GTGTTGC432000.023.1466482
Produced by FastQC (version 0.11.5)