FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79044820
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG19813350.082.3842851
GTGTTGG20575900.078.9868242
TGTTGGG20777150.076.9874043
GTTGGGT21682000.073.4094
TTGGGTG24293500.065.901155
TGGGTGT24109200.065.8894966
GGGTGTG25128800.063.0243647
GGTGTGT26769250.058.983328
GTGTGTT27752800.056.670829
GTTGCGT86700.039.050964
TGTTGCG105400.031.4502683
TTGCGTG118450.029.1411465
TGTGTTT28128650.027.74613810-11
GTGTTTG30943050.026.82393510-11
GTTTGGT19237850.025.28963712-13
TGTTTGG30883950.025.01819412-13
TTGGTTT11112900.022.5103814-15
TTTGGTT14017000.022.0189414-15
GTTTGGG14390750.021.40956512-13
TTGGTTG3649100.020.04573614-15
Produced by FastQC (version 0.11.5)