FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46826539
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG10308950.077.310741
GTGTTGG10570550.073.662352
TGTTGGG10792250.070.880143
GTTGGGT11478350.066.483514
GTTGCGT116300.064.4690864
TGGGTGT12546600.061.3303156
TTGGGTG12710000.060.850655
TGTTGCG121700.060.44993
GGGTGTG13149700.058.543077
TTGCGTG136800.055.767855
GGTGTGT14071050.054.2600568
GTGTGTT14694150.051.474189
GCGTGTG172050.048.5562257
CGTGTGT182250.044.137458
TGCGTGT206200.039.3584636
GTGTCGG44700.028.7537672
TGTCGGG45900.027.5172733
TGTGTCG63550.026.3131471
GTGTGTC410600.025.7675159
TGTGTTT14502750.025.75333210-11
Produced by FastQC (version 0.11.5)