FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16280926
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG337000.086.731583
CTTGCGT69300.077.063781
TTGCGTC57300.073.314142
GTGTTGC435050.069.814592
TGCGTCT61600.068.461713
TGTGTTG3671450.067.282671
GTTGCGT478250.062.3902974
GCGTGTC44300.061.941317
TTCGGTC22200.061.7654233
GTCTTGG261200.061.6447941
TTGGGTC420000.059.7209852
GTTCGGT42850.058.206232
TGGGTCT433950.057.7992443
AGTTGCG18700.057.703123
TCGGACG5150.057.641963
TTGCGTG522550.056.7715875
TCTTGGG288750.054.9939272
TGCGTGT553400.054.976426
CTGTTGG694600.053.2033961
CTTCGGT20450.053.0272522
Produced by FastQC (version 0.11.5)