FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16280926
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG442000.084.2777561
ATTGGGT844050.082.958473
TATTGGG493550.082.005692
AATTGGG368100.080.963252
GAATTGG372950.080.513291
TCTTGGG708700.076.828382
TTGAGTG657500.074.83912
GGATGTG634200.074.2221451
ATGAGTG416700.073.970712
GTTGAGT543000.073.437531
GATGAGT376400.073.43681
GTCTTGG511450.072.649631
TGAGTGT1112850.071.647033
GGATCTG236200.070.685951
GATCTGT288850.070.2884752
CTTGCGT74850.070.099111
CTCTTGG340100.068.97441
GAGTGTG1002800.068.056694
ATCTGTT340850.067.252993
GATGTGT758700.066.679892
Produced by FastQC (version 0.11.5)