FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22372386
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG535950.087.346243
CTTGCGT95050.074.424881
TTGCGTC82600.072.720492
GTGTTGC685650.071.8727652
TGTGTTG5202200.070.4429551
TGCGTCT89300.067.592473
GTCTTGG449200.067.57461
GCGTGTC63900.066.3626257
GTTGCGT739350.064.616934
TTCGGTC32600.061.717863
GTTCGGT56250.060.8529322
TCTTGGG475100.060.377012
TTGCGTG806450.058.914425
CTGTTGG1134200.058.2306481
TGCGTGT840950.058.0632176
GCGTCTG106450.057.13424
TTGGGTC556200.056.3204422
TGGGTCT568400.055.2020873
AGTTGCG26500.054.409673
TCTGTTG696500.052.8996161
Produced by FastQC (version 0.11.5)