FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5306235
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG336950.089.145811
TATTGGG377050.089.109862
ATTGGGT424150.086.724523
CTATTGG81400.078.896871
TGAGTTG275950.078.269781
GATCTGT129150.077.8200762
GGATCTG104800.076.623171
TATAGGG51000.074.7084962
CGATCTG23500.074.623311
CTTGCGT53200.074.5872041
AGCGTGT32750.074.387683
AGTTGGG296550.073.090723
GTATAGG50000.072.68471
TAGCGTG26950.071.9995962
GAGTTGG294750.071.4241942
ATCTGTT157500.071.242523
ATAGGGT68950.070.940033
ATCGGTG18450.070.764893
GTAGCGT21000.070.298151
ATCGGGT9300.070.1942143
Produced by FastQC (version 0.11.5)