FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6682157
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG76200.079.7219243
GTGTAGG335600.076.157581
TGTAGGG305100.075.422452
AGTTGCG17200.074.901823
GAGTAGG256050.070.1827551
AGTAGGG235450.069.972272
TGAGTTG183100.066.498351
AGATGCG13900.064.608373
TGATGCG17650.063.1355363
TTGCGTC16150.061.102572
GTTGCGT123150.059.060014
GTTCGGT20400.058.047442
GAAGGGT319350.057.6830331
TGTGATG248600.056.6841471
AAGGGTG324950.056.4988442
GATGCGT35350.055.5935554
GTGATGG233650.055.306942
GTAGGGT721350.054.4656373
GAGAGTT110150.054.3924182
GTAGGCT56100.053.9750673
Produced by FastQC (version 0.11.5)