FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68350435
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG24334400.066.665861
GTGTTGG26511950.061.1364822
TGTTGGG28455050.055.6769683
GTTGGGT30623150.051.4318164
TTGGGTG34587450.045.9775285
TGGGTGT34642150.045.623876
GGGTGTG38308000.041.1025057
GGTGTGT43292550.035.9582638
GTGTGTT44802050.034.6061029
GTGTTTG45684800.019.32613210-11
GTTTGGT26777500.018.72588212-13
GAACGAC212700.018.52527694
ACGACAT155750.018.49822494
TTGGTTT13790000.018.38565414-15
TGTGTTT41882450.018.37323610-11
TGTTGGT2276350.016.6415023
TGTTTGG46322900.016.6317912-13
AACGACA207900.016.57537394
GTTCGGT56650.016.424164
CGACATG129750.016.3997794
Produced by FastQC (version 0.11.5)