FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54511571
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG19857550.071.2229461
GTGTTGG21198950.066.594772
TGTTGGG22108000.062.4165653
GTTGGGT23271450.058.9033784
TTGGGTG26511050.052.000095
TGGGTGT26903250.050.9419026
GGGTGTG29669200.046.0537387
GGTGTGT32234250.042.288168
GTGTGTT32683950.041.4800959
GTTGCGT70600.024.3150844
GTGTTTG32170150.023.04106910-11
TGTGTTT30490000.021.99777610-11
GTTTGGT19586950.021.83018112-13
TGTTTGG32218100.020.64999412-13
GTGTCGG81400.020.2125512
TTGGTTT10209000.020.20119914-15
GTTTGGG14791150.020.00633412-13
TGTCGGG82450.019.6106573
TGTTGCG86200.019.2482723
TGTTGGT1663550.018.9927123
Produced by FastQC (version 0.11.5)