FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72488426
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG16657300.077.221941
GTGTTGG17386650.073.773562
TGTTGGG18034750.069.9498753
GTTGGGT18890500.066.411194
TGGGTGT20618450.060.748396
TTGGGTG20759800.060.7461365
GGGTGTG21976800.056.7875067
GGTGTGT23740750.052.3978278
GTGTGTT24690900.050.2869039
GTTGCGT64600.029.337194
TGTGTTT23830050.025.8558210-11
GTGTTTG25907250.025.8523310-11
CGATCCG32100.024.35523474-75
TGTTGCG79050.024.1093623
GTTTGGT14913500.023.54169812-13
TGTTTGG26121450.023.43168812-13
GTTTGGG11805200.020.82399412-13
TTGCGTG94550.020.3645385
TTGGTTT8973800.020.27913514-15
TTTGGTT11050550.019.63101214-15
Produced by FastQC (version 0.11.5)