FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87666652
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG14269050.082.813071
GTGTTGG14610050.080.603932
TGTTGGG14757650.079.537483
GTTGGGT14948400.078.477724
TGGGTGT15763450.074.611126
GGGTGTG16004450.073.3910147
TTGGGTG16364850.072.216265
GGTGTGT16277400.072.067228
GTGTGTT16761250.069.986789
TGTGTTT17471700.033.4296810-11
GTGTTTG19118900.032.04706610-11
TGTTTGG19196600.030.28573412-13
GTTTGGG9216400.029.28701412-13
TTGGGGG3787300.026.67963414-15
GTTGCGT60450.025.8425964
TTTGGGG5810800.025.42226814-15
GTTTGGT12094450.024.36854212-13
TTGGGGT2199700.022.7657514-15
TTTGGTG4791000.021.685114-15
TGGGGGG3184850.021.6734616-17
Produced by FastQC (version 0.11.5)