FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81621480
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG23229100.088.771361
GTGTTGG24267950.084.516172
TGTTGGG24739050.082.80673
GTTGGGT25287750.081.01344
GTGTGTT26275000.078.128159
TGGGTGT26379950.077.872836
GGTGTGT26457050.077.481588
GGGTGTG26557100.077.345517
TTGGGTG27123100.075.642915
GTTGCGT122050.046.724964
TGTTGCG132350.042.1577763
GTGTTTG27032000.039.97138610-11
TTGCGTG145150.039.3173835
GTTTGGT16467500.038.68680612-13
TGTTTGG26746100.038.16643512-13
TGTGTTT26898100.038.14460410-11
GTTTGGG12054400.035.06770712-13
TTTGGTT11921350.033.22297714-15
TTGGTTT9424000.032.80148314-15
TTTGGTG6673000.030.86037814-15
Produced by FastQC (version 0.11.5)