FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68249814
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG26511550.089.4623261
TGTTGGG27207100.086.774823
GTGTTGG27252550.086.706432
GTTGGGT27356900.086.322684
GTGTGTT28059700.084.1099
TGGGTGT28543100.082.7995766
GGTGTGT28548350.082.604598
GGGTGTG28864650.081.870567
TTGGGTG29422550.080.386955
GTTGCGT98700.052.768024
GTGTTTG28803400.042.08223310-11
TGTGTTT28349850.041.50231610-11
TGTTTGG28493350.041.08626612-13
TTGCGTG131700.039.6958475
GTTTGGT15758100.039.6427112-13
TGTTGCG132900.039.023863
GTTTGGG13873300.038.9018212-13
TTGGGGG5779500.034.66685514-15
TTTGGGG8634400.034.57360514-15
TTTGGTT11022400.034.5056414-15
Produced by FastQC (version 0.11.5)