FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56132193
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12111900.079.324651
GTGTTGG12444650.076.838212
TGTTGGG12582450.074.694063
GTTGGGT13399400.069.812444
TTGGGTG14980550.062.9775545
TGGGTGT14925550.062.908716
GGGTGTG15707050.059.6193357
GGTGTGT16866550.055.2465328
GTGTGTT17738600.052.152679
GTTGCGT52050.032.7612084
TGTTGCG68000.026.0104923
TGTGTTT17999150.025.54798310-11
TTGCGTG72800.024.1932375
GTGTTTG20527800.024.02538110-11
TGTTTGG20562800.022.14437112-13
GTTTGGT13542850.021.83321812-13
GTCGGGT38400.019.3974724
TTGGTTT8435100.019.23718514-15
TTTGGTT10282650.018.98754914-15
GTTTGGG9351900.018.38844512-13
Produced by FastQC (version 0.11.5)