FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67801981
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG14027450.078.992361
GTGTTGG14373650.076.667822
TGTTGGG14565350.074.447453
GTTGGGT15517400.069.536174
TTGGGTG17422350.062.4557725
TGGGTGT17346300.062.40626
GGGTGTG18234900.059.2428747
GGTGTGT19477900.055.1609738
GTGTGTT20482300.052.154569
GTTGCGT68150.039.1145974
TGTTGCG85600.031.6249353
TTGCGTG86800.031.030415
TGTGTTT20866100.025.4675910-11
GTGTTTG24009400.023.61051610-11
TGTTTGG24125850.021.80447812-13
TGCGTGT132550.021.7777186
GTTTGGT15738300.021.2435212-13
GCGTGTG145500.020.1826047
TTGGTTT9900750.018.82259814-15
GTGTCGG46400.018.6154082
Produced by FastQC (version 0.11.5)