FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70146336
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG17723850.081.698781
GTGTTGG18340000.078.65582
TGTTGGG18526450.076.539323
GTTGGGT19493300.072.40774
TTGGGTG21821050.065.128755
TGGGTGT21658750.065.0513156
GGGTGTG22760800.061.7599377
GGTGTGT24066900.058.1813668
GTGTGTT24899550.056.1902479
GTTGCGT83050.028.0245484
TGTGTTT25101050.027.65585710-11
GTGTTTG28200650.026.23149910-11
TTGCGTG96850.024.394325
TGTTTGG28271550.024.36000812-13
GTTTGGT17398550.024.24764412-13
TTGGTTT10241900.021.44873614-15
TTTGGTT12636700.021.12065514-15
GTTTGGG13308600.020.6700912-13
TTTGGTG6139450.019.71278414-15
TTGGGTT3473950.019.49383514-15
Produced by FastQC (version 0.11.5)