FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45265244
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12186450.082.0242541
GTGTTGG12669000.078.622672
TGTTGGG12800700.076.447283
GTTGGGT13447800.072.396154
TGGGTGT14995050.064.790836
TTGGGTG15141250.064.753375
GGGTGTG15722250.061.651937
GGTGTGT16587150.058.2167138
GTGTGTT17177650.056.1592459
GTTGCGT46650.034.6001854
TTGCGTG58750.027.9588345
TGTGTTT17249500.027.75301210-11
TGTTGCG62300.026.6488933
GCGCGTC25950.026.18292618-19
GTGTTTG19668200.026.02270110-11
TGTTTGG19794100.024.01994712-13
GTTTGGT12164150.023.98893212-13
CGCGTCG40450.023.82994518-19
TTGGTTT7113050.021.46696314-15
TTTGGTT8821000.021.00092514-15
Produced by FastQC (version 0.11.5)