FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65154892
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12166250.080.483241
GTGTTGG12536700.077.767452
TGTTGGG12698650.075.584783
GTTGGGT13534150.070.576744
TTGGGTG15202700.063.4687735
TGGGTGT15162750.063.225436
GGGTGTG15989750.059.8310627
GGTGTGT17197350.055.4385158
GTGTGTT18174550.052.4307149
GTTGCGT61950.034.8665924
TGTTGCG77950.028.6094283
TTGCGTG80450.027.579995
TGTGTTT18546200.025.5633110-11
GTGTTTG21283900.023.7893810-11
TGTTTGG21417000.022.00538312-13
GTTTGGT13860950.021.24642812-13
TTGGTTT8494500.018.70928414-15
TTTGGTT10441000.018.3828714-15
GTTTGGG10116950.018.09817112-13
TGCGTGT135350.017.765176
Produced by FastQC (version 0.11.5)