FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57415136
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG11096550.079.730681
GTGTTGG11441450.077.015932
TGTTGGG11580300.074.768093
GTTGGGT12276100.070.183424
TTGGGTG13711350.063.349355
TGGGTGT13705700.062.8854686
GGGTGTG14436050.059.5235527
GGTGTGT15645750.054.734918
GTGTGTT16479150.051.825089
GTTGCGT60900.035.6277284
TGTTGCG74550.029.2144283
TTGCGTG78600.028.0222345
TGTGTTT16828000.025.25252510-11
GTGTTTG19196200.023.69664410-11
TGTTTGG19268550.021.87205512-13
GTTTGGT12608250.021.35625812-13
TTGGTTT7884800.018.81424114-15
TGCGTGT125350.018.6047176
TTTGGTT9613450.018.40728814-15
GTTTGGG8832850.018.12028512-13
Produced by FastQC (version 0.11.5)