FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16605776
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTGGGTGTGTTTGGTTGTTGTTTTACAAGGACTACTGGAACTGTCA179090.10784801625651219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCGT322450.084.65544
TGTTGCG306400.084.387393
TTGCGTG334500.082.474175
GCGTGTG384850.079.051117
CGTGTGT391000.077.817958
GTGTTGC365700.071.861852
TGTGTTG4601400.069.223751
GATGCGT26300.068.477224
GTGTTGG4364150.064.594222
AGTTGCG25100.064.112153
TGTTGGG4497150.061.269963
TGATGCG17450.057.7722553
ATGCGTG31850.057.3800775
GTTGGGT4832900.057.1871834
AGATGCG13850.056.7274783
TGGGTGT5100000.055.1582766
TTGGGTG5093300.055.0633935
TGCGTGT562250.053.2398456
GGGTGTG5322200.053.1639487
TTGCGGG30700.049.7101945
Produced by FastQC (version 0.11.5)