FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40567614
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGG1809250.086.7209558
CTAAGGA1718550.086.074369
TCCTAAG1912800.082.183527
CTTCCTA1982450.081.9321755
TTCCTAA1987850.080.372826
TGTGTTG7631400.079.794941
GTCTTCC2062800.079.454993
GTGTTGG7854850.077.235312
TGTTGGG7939500.075.088993
TGTCTTC2205600.073.924382
TCTTCCT2280400.071.323184
GTTGGGT8407650.070.541074
TTGTCTT2313700.069.824891
TTGGGTG9523800.062.7967875
TGGGTGT9502100.062.413466
GGGTGTG9959100.059.393747
GGTGTGT10702500.055.1116528
GTGTGTT11224350.052.3873029
CCTAACG47650.049.051738
CTAACGA40500.047.3889129
Produced by FastQC (version 0.11.5)