FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22634570
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGG939450.087.3002248
CTAAGGA918050.085.365299
TCCTAAG994400.082.904287
CTTCCTA1053600.082.8710255
GTCTTCC1078900.081.686593
TTCCTAA1072500.080.621766
TGTGTTG3675300.078.869971
GTGTTGG3749150.075.916852
TGTCTTC1164550.075.73652
TCTTCCT1184300.073.749614
TGTTGGG3799800.073.1274263
TTGTCTT1231050.071.07411
GTTGGGT4073250.067.767934
CCTAACG34700.063.3291978
CTAACGA31650.061.2675939
TTGGGTG4734400.058.9544535
TGGGTGT4732650.058.3769536
GGGTGTG5045900.054.572397
GTTGCGT45950.050.171584
GGTGTGT5495500.049.849318
Produced by FastQC (version 0.11.5)