FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22634570
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG6140650.083.3224261
GTGTTGG6301500.080.3009342
TGTTGGG6368050.078.04663
GTTGGGT6695700.073.836284
TTGGGTG7525600.066.101525
TGGGTGT7494650.065.883836
GGGTGTG7797850.063.135287
GGTGTGT8336850.058.857928
GTGTGTT8643900.056.4708379
GTTGCGT42650.054.3946654
TTGCGTG49650.047.4506575
TGTTGCG48450.047.391163
GCGTGTG75350.032.8210267
TGCGTGT78750.030.8940496
CGTGTGT83150.028.151578
CGCGTCG20400.027.66523718-19
TGTGTTT8774350.027.62941210-11
GTGTTTG9852000.026.25514610-11
GCGCGTC13100.024.9515118-19
TGTTTGG9862100.024.32356312-13
Produced by FastQC (version 0.11.5)