FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19399905
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG3754100.071.589581
GTTGCGT88850.070.1437154
TGTTGCG92600.067.866813
GTGTTGG3799750.066.995942
TGTTGGG3940700.063.3095443
TTGCGTG104400.060.1947865
GTTGGGT4276150.058.1223874
TGGGTGT4752650.052.929696
TTGGGTG4766550.052.89155
GCGTGTG121750.052.5711067
TGCGTGT123800.052.1797686
CGTGTGT122200.051.3350948
GGGTGTG5169600.048.569127
GGTGTGT5637800.044.162268
GTGTGTT6061050.041.4340029
GTGTTGC174850.036.4068262
GTGTGTC175800.026.6977759
GTTTGCG36250.021.898693
GTTGAGT113850.021.3158324
TGTGTTT5876600.021.17507710-11
Produced by FastQC (version 0.11.5)