FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19399905
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG4808850.075.871341
TGTCGGG70200.072.873653
GTGTTGG4892200.072.351092
GTGTCGG76000.070.03672
TGTTGGG5001600.069.349193
GTTGGGT5349400.064.780444
TGTGTCG88350.063.2874761
TCGGGTG81050.062.136895
GTTGCGT72100.060.48624
CGGGTGT83250.059.9748956
TGGGTGT5943350.059.3387156
TTGGGTG5956400.058.9349635
TGTTGCG75100.056.335163
GGGTGTG6371800.056.101917
GGTGTGT6840200.052.3684588
TTGCGTG87000.050.4686245
GTGTGTT7235500.049.7047659
GCGTGTG108350.044.079317
GTCGGGT115550.043.168364
CGTGTGT110700.042.220868
Produced by FastQC (version 0.11.5)