FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19880450
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG5394400.070.1348651
GTGTTGG5413150.068.007882
TGTCGGG62100.066.983773
GTGTCGG65350.065.4740752
TGTTGGG5553500.064.971453
GTTGGGT5844700.061.1069564
TGTGTCG75300.060.0008281
TGGGTGT6147900.059.0402156
TTGGGTG6142100.058.7885785
GTTGCGT72750.056.8339964
GGGTGTG6459500.056.7333227
TCGGGTG64600.056.6575555
TGTTGCG73500.056.272533
CGGGTGT65100.054.7140276
GGTGTGT6993600.052.2885748
TTGCGTG81850.051.450335
GTGTGTT7464250.049.2681669
GCGTGTG98200.046.42437
GGGAGTG139100.044.1383867
CGTGTGT106650.041.7009858
Produced by FastQC (version 0.11.5)