FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15263912
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197250.12922637394660033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG3250900.058.9690441
GTGTTGG3315850.055.8041842
GTTGCGT51500.055.113534
TGTTGGG3392900.053.4687273
TGTTGCG55550.051.793223
TTGCGTG57050.051.0757455
GTTGGGT3668000.049.309384
TGGGTGT3952700.046.914396
TTGGGTG3937250.046.7805755
GGGTGTG4214200.043.9428147
GCGTGTG76950.040.3571437
GGTGTGT4597250.039.1760448
TGCGTGT85450.037.070026
CGTGTGT80350.036.4914938
GTGTGTT4912250.035.7734539
GTGTTGC108100.026.95232
GTGTGTC171350.025.057099
GTTTGCG21850.023.7837833
TTGGTTT2736300.023.0630873
TGGTTTT2383150.022.9773224
Produced by FastQC (version 0.11.5)