FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24747773
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCGT101900.065.893844
TGTGTTG4680050.064.9267351
TGTTGCG105350.064.0875243
TTGCGTG110400.061.5912975
GTGTTGG4740200.060.7228282
TGTTGGG4931850.057.2415123
GTTGGGT5335550.052.7148484
GCGTGTG134050.051.8237657
TGCGTGT144100.049.007656
TTGGGTG5839150.048.900045
TGGGTGT5832850.048.863196
CGTGTGT142400.047.8463868
GGGTGTG6325700.044.9962467
GGTGTGT7047900.039.7357528
GTGTGTT7610650.036.3656739
GTGTTGC198150.034.7149932
GTGTGTC259600.030.9433219
GTTTGCG36600.025.4308533
TTGGTTT3992950.021.2407843
TGGTTTT3516800.020.609314
Produced by FastQC (version 0.11.5)