FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34799467
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCGT148100.073.758974
TGTTGCG157000.070.2013
TGTGTTG6178100.069.271911
GTGTTGG6156950.065.3455662
TTGCGTG177900.062.1740235
TGTTGGG6368400.061.984023
GTTGGGT6807350.057.6947984
GCGTGTG197950.057.0168887
TTGGGTG7262700.054.7977755
TGGGTGT7256900.054.7262736
CGTGTGT205450.053.2099278
GGGTGTG7682300.051.650577
TGCGTGT222950.051.4209636
GGTGTGT8516150.045.935458
GTGTGTT9253050.041.893569
GTGTTGC274100.040.5634732
GTGTGTC350150.034.5666859
GTTTGCG52900.025.3733043
TGTGTTT8992350.021.29285610-11
GTTGAGT185800.020.1560864
Produced by FastQC (version 0.11.5)