FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53893399
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG11858300.077.06911
GTGTTGG12236900.073.437532
TGTTGGG12531100.070.386993
GTTGGGT13222350.066.42544
TGGGTGT14485400.060.890516
TTGGGTG14662250.060.556785
GTTGCGT127700.057.9041944
GGGTGTG15393400.057.1955647
TGTTGCG134200.054.292763
GGTGTGT16707800.052.52268
TTGCGTG149350.050.0117725
GTGTGTT17623300.049.8287129
GCGTGTG199500.039.6838577
TGCGTGT209150.037.244976
CGTGTGT210900.036.790048
TGTGTTT17520950.024.88381610-11
GTGTCGG46300.024.0847052
GTGTTTG19715800.023.92566710-11
TGTCGGG46700.022.590993
TGTTTGG19798900.021.70533612-13
Produced by FastQC (version 0.11.5)