FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21193958
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTCGGATCGTAGCCATGTCGTTCCTTAGGAAGACAAAAGTCGGATCGT622280.2936119813014634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG4917600.080.9705661
GTGTTGG5077350.077.199032
TGTTGGG5140200.074.632843
GTTGGGT5451900.069.992534
TTGGGTG6155900.062.6087075
TGGGTGT6161700.062.2226566
GGGTGTG6541050.058.606797
GTTGCGT59550.055.1515464
GGTGTGT7017100.054.3563168
GTGTGTT7323350.051.7590529
TGTTGCG64400.050.363893
TTGCGTG67750.049.3442965
GCGTGTG96900.037.1609347
TGTCGGG28450.031.7230533
GTGTCGG30000.031.3465332
GCGCGTC18100.027.59989718-19
CGCGTCG31750.027.01517718-19
TGTGTCG41400.026.579441
GTTCGCG19650.025.69333816-17
TGTGTTT7396000.025.45180910-11
Produced by FastQC (version 0.11.5)