FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15810073
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCTTCCTAAGGAACGACATGGCTACGATCCGACTTTTGTCTTCCTAA520690.3293406678134883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGA1831500.088.677479
CCTAAGG1927750.087.303758
TCCTAAG1972650.085.321277
TTCCTAA2017700.085.128536
CTTCCTA2065250.084.769565
GTCTTCC2192600.080.926263
TCTTCCT2210950.079.186014
TGTCTTC2238950.078.532042
TTGTCTT2313450.075.545831
TGTGTTG1989550.073.5506441
CCTAACG50150.070.186898
CTAACGA45100.069.869879
GTGTTGG2076500.069.789752
TGTTGGG2112100.067.0332643
GTTGGGT2267400.062.1291244
CCGAAGG39750.060.9670878
TCCGAAG41750.057.639017
TTCCGAA39700.056.2565736
CGAAGGA41500.054.139059
TTGGGTG2694100.053.1741645
Produced by FastQC (version 0.11.5)