FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15810073
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTCGGATCGTAGCCATGTCGTTCCTTAGGAAGACAAAAGTCGGATCGT428900.2712827448677815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG3499100.081.028541
GTGTTGG3616900.077.158622
TGTTGGG3673000.074.510943
GTTGGGT3884350.070.1444854
TTGGGTG4395950.062.6093565
TGGGTGT4434800.062.0266726
GTTGCGT41750.059.3263634
GGGTGTG4724650.058.3162277
GGTGTGT5058300.054.0242048
TGTTGCG46000.053.614213
TTGCGTG48950.051.6243865
GTGTGTT5216950.050.798329
GCGTGTG69300.039.6942257
CGTGTGT79400.034.1090858
TGCGTGT80500.033.852346
TGTCGGG20700.032.80413
GTGTCGG23250.031.1634542
GTGTGTC160150.029.3612949
TGTGTCG29950.028.457421
GCGCGTC12400.028.307318-19
Produced by FastQC (version 0.11.5)