FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56557953
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12778600.077.6093751
GTGTTGG13060100.074.653572
TGTTGGG13356500.071.716183
GTTGGGT14024000.068.017474
TGGGTGT15157850.063.2243236
TTGGGTG15399200.062.6800275
GGGTGTG15823500.060.4347427
GTTGCGT137600.059.0199784
TGTTGCG145800.055.7335133
GGTGTGT17204750.055.3973588
GTGTGTT18283850.052.134639
TTGCGTG181900.045.4771655
GCGTGTG203400.043.288497
CGTGTGT223050.038.783258
TGCGTGT228000.037.9565286
TGTGTTT18321000.025.78883710-11
GTGTTTG20896700.024.25636510-11
GTGTCGG46950.024.0525282
TGTCGGG50050.022.306383
TGTTTGG21051900.022.11412612-13
Produced by FastQC (version 0.11.5)