FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20882147
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCGT113800.067.820434
TGTTGCG116900.066.441183
TTGCGTG120150.064.474145
TGTGTTG6745500.060.1658861
GTGTTGG6822000.056.772822
GCGTGTG140800.055.7178047
TGTTGGG7015800.054.0442353
CGTGTGT149150.051.6523978
GTTGGGT7523800.050.1797754
TGGGTGT7697650.049.6339656
TTGGGTG7736400.049.568115
GGGTGTG8015300.047.4714057
GGTGTGT8736550.043.2150428
GTGTTGC182000.043.056772
GTGTGTT9396750.040.3812879
TGCGTGT198200.040.376496
GTGTGTC210950.030.324199
GTTTGCG38550.025.2613813
TTGCGGG19400.023.3741055
TTTGCGG32150.022.7720154
Produced by FastQC (version 0.11.5)