FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94858336
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG23571100.055.5358161
GTGTTGG24880000.052.5318452
TGTTGGG25229750.051.1621443
GTTGGGT26802100.047.9816674
TGGGTGT28946400.044.745716
TTGGGTG29673250.044.2632035
GGGTGTG30227250.042.5825237
GGTGTGT33599700.038.1644068
GTGTGTT36094150.035.470359
TTGGTTT17447850.024.0577663
TGGTTTT15311650.023.9033724
TTTGGTT20863250.022.452862
GGTTTTT15422500.020.6010465
GAACGAC212950.020.01071494
GTTTGGT27251600.019.4866661
CGACATG125400.018.91694
TGTGTTT33824100.018.70121810-11
GTGTTTG39755400.017.42658610-11
ACGACAT160450.016.71216294
TGTTTGG39766250.015.9379962
Produced by FastQC (version 0.11.5)