FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49901250
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12032300.070.3956451
GTGTTGG12677950.066.462842
TGTTGGG13139600.062.7500763
GTTGGGT13951400.058.8347664
TTGGGTG15849500.052.5606235
TGGGTGT15761400.052.3131456
GGGTGTG16928350.048.4733737
GGTGTGT18513300.044.130448
GTGTGTT19317150.042.07669
GTTGCGT52500.029.0324974
TGTTGCG63800.023.3455143
TTGCGTG68650.022.6154125
CGACTTA36850.022.52034278-79
TGTGTTT18579900.021.69282210-11
GTGTTTG21180200.020.94471210-11
TGTTTGG21488150.018.65493412-13
GTTTGGT13972450.018.4359112-13
AAGACCG219350.017.03549694
CCGACTT55050.017.00211778-79
GTTTGGG10291100.016.54962712-13
Produced by FastQC (version 0.11.5)