FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61160701
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG21648350.068.819621
GTGTTGG22873200.064.835732
TGTTGGG24263000.059.7378123
GTTGGGT25763050.055.9263534
TTGGGTG29215700.049.790375
TGGGTGT29419450.049.0820356
GGGTGTG31615900.045.5036057
GGTGTGT34880950.041.133828
GTGTGTT36077150.039.63249
GTTGCGT71900.036.2760434
TGTTGCG85200.030.396523
TTGCGTG87350.029.8508785
GTGTTTG37115100.021.18952410-11
TGTGTTT34018650.020.7902410-11
TGCGTGT132500.020.1131676
GTTTGGT23500950.019.98953212-13
GCGTGTG143200.019.287747
TTGGTTT12948450.018.91002514-15
TGTTTGG37571800.018.72745712-13
AAGACCG228850.018.61203294
Produced by FastQC (version 0.11.5)