FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57252104
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG22831450.071.754061
GTGTTGG24222150.067.26762
TGTTGGG25834100.061.7185173
GTTGGGT27311150.058.1324734
TTGGGTG30848100.051.9945765
TGGGTGT30846850.051.6591646
GGGTGTG33329450.047.6626787
GTTGCGT96450.044.1559684
GGTGTGT37371150.042.3531958
GTGTGTT38570700.040.7259189
TGTTGCG105900.039.3474853
TTGCGTG116700.036.879995
GCGTGTG168550.027.1516867
TGCGTGT167300.026.7064366
CGTGTGT187950.023.0006758
GTGTTTG39386600.022.02486210-11
GTTTGGT24455550.021.4045412-13
TGTGTTT36542800.021.25050210-11
TTGGTTT13116850.020.5467314-15
TGTTTGG39711050.019.5122612-13
Produced by FastQC (version 0.11.5)