FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005752496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77742370
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG19854450.069.689161
GTGTTGG21135050.065.227832
TGTTGGG22181900.061.0121653
GTTGGGT23759850.056.6793634
TTGGGTG26379450.051.6904265
TGGGTGT26238650.051.495996
GGGTGTG27942400.048.1142277
GGTGTGT30803150.043.281128
GTGTGTT32268600.041.0227369
GTTGCGT81750.030.522314
TGTTGCG95350.026.724773
TTGCGTG112600.022.5889575
TGTGTTT30924700.021.30442410-11
GTGTTTG35282850.020.83594910-11
GTTTGGT23357500.018.46718212-13
TGTTTGG35892250.018.29186212-13
ACGACAT222150.017.97187894
GAACGAC303850.017.83887394
CGACATG182800.017.18979894
CGTAGCC29300.016.67195794
Produced by FastQC (version 0.11.5)