FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54208977
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG12919400.062.493321
GTGTTGG13467400.059.569092
TGTTGGG13759700.057.224833
GTTGGGT14549200.053.8520284
TGGGTGT15793500.049.83486
TTGGGTG16031750.049.6528245
GGGTGTG16422100.047.677787
GGTGTGT17828450.043.7416468
GTGTGTT18839750.041.1450049
GTTGCGT70450.036.2984964
TGTTGCG77500.033.1969153
TTGCGTG82150.031.8943375
TGCGTGT115900.023.4041656
CGATCCG29300.023.28672474-75
GCGTGTG121150.022.3875397
ATCCGAC30050.021.93248276-77
TGTGTTT17782250.021.66855810-11
GTGTTTG21019450.020.00286910-11
CGTGTGT136300.019.7389648
ACGACAT235600.019.51717694
Produced by FastQC (version 0.11.5)