FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20335873
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG3692550.082.7435461
GTGTTGG3787500.077.9181062
TGTTGGG3911450.075.2547763
GTTGGGT4079900.071.994514
GTTGCGT91050.069.793144
TGGGTGT4475950.067.758076
GGGTGTG4604250.066.383347
TTGGGTG4485550.065.960035
TGTTGCG97550.065.4537663
GGTGTGT4639900.065.180678
TTGCGTG100400.063.6533435
GTGTGTT4704300.062.5567479
GCGTGTG127700.052.4355287
CGTGTGT133700.049.0398988
TGCGTGT173400.041.8604556
GTCGGGT27350.039.555584
GTGTTGC181300.035.5263332
GTGTCGG36750.030.8760532
TGTGTTT4867850.030.30611810-11
TGTGTCG41050.029.5770681
Produced by FastQC (version 0.11.5)