FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16255598
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG2350800.073.9755551
GTGTTGG2457300.068.353762
TGTTGGG2519300.064.691423
GTTGCGT52300.063.064634
GTTGGGT2765550.058.461034
TGTTGCG60200.055.280543
TTGCGTG62000.053.4117055
TTGGGTG3208750.050.827475
TGGGTGT3289550.050.3019456
GGGTGTG3748950.044.3316767
GCGTGTG82150.041.447497
GTCGGGT18450.039.0179634
CGTGTGT86700.038.6296968
GGTGTGT4364400.037.676358
TGCGTGT102150.035.0786826
GTGTGTT4745000.033.7273989
GTGTCGG25900.030.008742
TGTCGGG25500.029.3498783
TGTGTCG27900.028.6879041
GTGTGTC147800.026.0576579
Produced by FastQC (version 0.11.5)