FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14007384
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG2380850.068.866481
GTGTTGG2507500.063.5055662
GTTGCGT42400.061.351664
TGTTGGG2553800.060.7218063
GTTGGGT2793850.055.1276134
TGTTGCG47700.055.0426983
TTGCGTG49700.053.4631845
TGGGTGT3266700.048.515876
TTGGGTG3214500.048.3805055
GGGTGTG3661850.043.5579077
GTCGGGT17100.038.512264
GGTGTGT4173100.037.6679658
GCGTGTG75600.036.2766347
CGTGTGT77750.034.7986348
GTGTGTT4518550.033.611919
TGCGTGT96300.031.0520946
GTGTCGG23450.028.9150852
TGTTCGG16650.026.866692
AACGACG11200.026.0337355
TGTCGGG25850.025.6650683
Produced by FastQC (version 0.11.5)