FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14007384
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG3725850.078.952411
GTGTTGG3821850.075.2970662
TGTTGGG3853050.073.269083
GTTGGGT4075050.068.980774
TGGGTGT4504300.062.48266
TTGGGTG4530300.062.450485
GTTGCGT45850.060.1125764
GGGTGTG4802950.058.554157
TGTTGCG47750.058.3029753
TTGCGTG50200.055.4818735
GGTGTGT5288300.052.9418228
GTGTGTT5601200.049.9542439
GCGTGTG69850.043.447147
CGTGTGT73300.040.5180858
TGCGTGT85550.035.83966
TGTCGGG15800.026.1148853
TGTGTTT5599100.024.77115810-11
GTGTTTG6133100.024.41406810-11
TGTTTGG6074300.022.47564912-13
GTGTTGC129450.022.3916682
Produced by FastQC (version 0.11.5)