FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7427739
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG1248200.076.249141
GTGTTGG1273000.072.8663252
TGTTGGG1283150.070.578993
GTTGGGT1373700.065.467794
GTTGCGT21750.064.087894
TGTTGCG22450.061.9039733
TTGGGTG1579250.057.48245
TTGCGTG24550.057.3349535
TGGGTGT1591750.056.484536
GGGTGTG1726850.051.9254957
TGCGTGT30250.046.6854936
GGTGTGT1913500.046.6184738
GCGTGTG31050.045.4814227
GTGTGTT2038200.043.5396089
CGTGTGT32900.042.764028
GTGTTGC56300.025.5154552
TGTGTTT2058950.021.37158410-11
GTGTTTG2330400.020.65420510-11
GTTTGGT1454250.018.74154712-13
TGTTTGG2332250.018.7205512-13
Produced by FastQC (version 0.11.5)