FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7427739
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG1960350.080.8751141
GTGTTGG1994650.078.3176652
TGTTGGG2006700.076.2765053
GTTGGGT2113100.072.081084
TTGGGTG2363500.064.8835755
TGGGTGT2364850.064.470666
GTTGCGT20650.062.520094
GGGTGTG2480350.061.352757
TGTTGCG22600.057.3345383
GGTGTGT2660950.057.006428
GTGTGTT2764350.054.7748649
TTGCGTG25000.052.6483155
TGCGTGT31700.042.705626
GCGTGTG32400.042.395917
CGTGTGT34000.040.1650628
TGTGTTT2786400.026.92228510-11
GTGTTTG3075300.026.0721910-11
GTTTGGT1941050.024.37218512-13
TGTTTGG3069050.024.12342312-13
GTGTTGC62700.021.7809722
Produced by FastQC (version 0.11.5)