FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9820076
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG2975200.083.498741
GTGTTGG3043250.080.523682
TGTTGGG3073650.078.302663
GTTGGGT3236450.073.9343644
TTGGGTG3663750.065.71825
TGGGTGT3671200.065.19056
GTTGCGT32300.064.390074
GGGTGTG3838500.062.212247
TGTTGCG34750.060.3932043
GGTGTGT4008650.059.394468
GTGTGTT4086300.058.1406489
TTGCGTG37600.056.0040785
GCGTGTG47850.046.4006967
CGTGTGT48500.045.245218
TGCGTGT52550.041.2342156
GTTCGGT9750.031.7802264
TGTGTTT4096700.028.77025610-11
TGTTCGG10800.028.691653
GTGTTTG4537850.027.6908510-11
TTCGGTG11950.026.746725
Produced by FastQC (version 0.11.5)