FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005752532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005752532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5244716
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTTG958400.080.855451
GTGTTGG979600.077.186432
TGTTGGG996950.074.496063
GTTGCGT17550.071.144954
GTTGGGT1066500.069.174874
TGTTGCG18450.068.4645843
TTGCGTG19550.063.3664785
TTGGGTG1245600.059.79375
TGGGTGT1252800.059.0011566
GGGTGTG1338600.055.031477
GCGTGTG23450.053.6268047
GGTGTGT1418550.051.7483258
CGTGTGT24600.050.9067088
GTGTGTT1471200.049.7535069
TGCGTGT26000.048.3691026
GTGTTGC40550.031.3786622
TGTGTTT1486950.024.41500510-11
GTGTTTG1719250.022.77854710-11
TGTTTGG1727000.020.87638312-13
GTTTGGT1059600.020.44289212-13
Produced by FastQC (version 0.11.5)